Hoechst 33258 Solution

Code: 94403-1ml D2-231

Application

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quant...


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Application

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA. Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.

General description

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.

assay≥98.0% (HPLC)
concentration1 mg/mL in H2O
fluorescenceλex 355 nm; λem 465 nm in TE buffer; DNA
formliquid
InChI keySMNPLAKEGAEPJD-UHFFFAOYSA-N
InChI1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H
Quality Level100
SMILES stringCl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6
solubilityDMF: soluble, H2O: soluble
storage temp.2-8°C
Cas Number23491-45-4
This product has met the following criteria: