Application

Phosphatidylcholine Assay Kit has been used to determine the concentration of phosphatidylcholine in samples.

General description

Phosphatidylcholine (PC) accounts for more than 50% of the phospholipids composing mammalian plasma membranes and is particularly enriched in the extracellular leaflet. PC is synthesized in the liver and is required for lipoprotein assembly and secretion. PC is cleaved by phospholipase D, which hydrolyses the choline head, producing the signaling intermediate phosphatidic acid.

Principle

In this assay, PC concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λ_ex = 535/λ_em = 587 nm) product, proportional to the PC present.

Suitability

Suitable for the measurement of phosphatidylcholine in a variety of biological samples