Amersham ECL Western blotting detection kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL detection reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera. ECL Western blotting system is the world’s most widely used and referenced chemiluminescent immunodetection system.

• Convenient kit that includes ECL detection reagent, HRP-conjugated antibodies, and blocking agent.
• Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents.
• Optimized system provides rapid, sensitive results using simple protocols.
• Designed for use with nitrocellulose and PVDF membranes.
• High sensitivity: Up to 10 times more sensitive than colorimetric methods.
• Rapid: results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
• Easy reprobing: save time and materials by reprobing on membranes up to 10 times.
• Quantifiable: linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,
• Non-radioactive-no specialised facilities needed
• Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide