Application

Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture: living colon cancer SW620 cellshuman breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231mammal and canine derived adipose-derived mesenchymal stem cells

General description

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.

Other Notes

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.

Reconstitution

Supplement with 0.584 g/L L-glutamine.