Application

L-15 Medium (Leibovitz) has been used: in protein incorporation for incubation of high blastula embryos and to avoid the abnormal development of embryosas a supplement in sterile cell media for the dissection and isolation of kidney cellsas a component of culture buffer for the tissue culture of Newt lung cells

General description

L-15 Medium (Leibovitz) was initially prepared to be used in carbon dioxide (CO₂) free systems, which involves sodium bicarbonate. The complement of free base amino acids, salts, and galactose buffers it. This aids the maintenance of physiological pH control.

Reconstitution

Supplement with 0.3 g/L L-glutamine.