FGF-2 TOP® (formerly STAB) is a stabilised growth factor that offers a novel way to grow FGF-2-dependent cell cultures more efficiently, with fewer media changes. FGF-2 TOP® retains full biological activity even after twenty days. The stable level of FGF-2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving researchers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required.

Core Biogenesis FGF-2 TOP® is the 155 aa mature domain of FGF-2, tag-free with nine amino acid substitutions to enhance stability without impacting bioactivity developed by Dvorak et al. 2018. This increases the functional half-life of the protein from 10 h (wild-type) to >7 days (FGF-2 TOP®) in cell culture conditions at 37°C.

Superior thermostability:

• Offers a novel way to grow FGF-2-dependent cell cultures more efficiently, with fewer media changes
• Retains full biological activity even after twenty days at 37°C
• The stable level of FGF-2 in culture allows for a more homogenous, undifferentiated pluripotent stem cell cultures, and consistent downstream differentiations, while saving researchers valuable time and money because repeated supplementation with FGF-2 and a daily medium change is not required

Superior bioactivity:

• Expected ED50 of less than 0.1ng/mL, as determined by NIH-3T3 cell proliferation assay
• The ED50 value of FGF-2 TOP® is about 5-times lower than that of the wild-type FGF-2, as determined by a NIH/3T3 proliferation assay
• Under the same experimental conditions, less FGF-2 TOP® is needed to achieve a given effect on cell proliferation, thanks to the higher stability and prolonged half-life of FGF-2 TOP®
• Thanks to this competitive advantage, lower dosage of FGF-2 are required in your cell culture applications (up to 10x)

Details

• Accession number: 155 amino acids with 9 aa point mutations from the wild-type FGF-2, tag-free, original reference sequence accession number: P09038
• Origin: FGF-2 TOP® is produced in plant seeds of Camelina sativa cultivated in a facility in Strasbourg, France
• Species: engineered sequence, neutral species, reactivity with Human, Bovine and Porcine
• Purity: ≥ 90% measured by SDS page, and resolved under reduced (R) conditions
• Bioactivity: the biological activity of recombinant FGF-2 TOP® was determined by its ability to promote the proliferation of NIH/3T3 cells cultured in adherent condition, cell proliferation is measured using non-radioactive colorimetric assay and monitored by absorbance, EC50 is generally: ≤1ng/mL
• Endotoxin level: recombinant protein expressed in plant system, free of bacterial endotoxins
• Animal-derived component free. Core Biogenesis strictly guarantees that recombinant proteins are not produced with or contain any components of animal origin.
• Formulation: PBS at 1mg/mL or lyophilized