The MycoAlert™ Assay Control Set includes a lyophilized positive control and assay buffer for reconstitution. The assay buffer also serves as a negative control. The positive and negative controls are designed for use with the MycoAlert™ Mycoplasma Detection Kit. It is recommended to always include the Control Set to ensure that the operator and all of the assay reagents are performing properly. The positive control is proprietary, but does NOT contain mycoplasma. The positive or negative control is simply substituted for a sample in the standard assay protocol.