Support non-enzymatic harvesting of adherent cells for preservation of cell viability and surface proteins with the temperature-responsive surface of Thermo Scientific™ Nunc™ UpCell™. Simply transfer the culture to room temperature to collect cells in suspension. UpCell surface is a temperature-responsive cell culture surface that enables non-enzymatic harvesting of adherent cells for preservation of cell viability and surface proteins. A temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) polymer is grafted onto the standard cell culture treated polystyrene surface. At 37°C, the covalently bound polymer allows cells to attach to the dish and grow as normal. At room temperature, the PNIPAAm binds water and swells, releasing the attached cells together with the extracellular matrices. The cells are dissociated from the culture substrate without trypsin.

• The hydrophicility of the UpCell surface changes with the external temperature, allowing cells to detach
• No need for dissociation enzymes, ensuring intact cell surface proteins
• No need for physical scraping, resulting in high cell viability