Fatty acid-free BSA is used where the presence of lipids and fatty acids may cause problems, such as hormone and cholesterol assays. Fatty acid free albumin also provides maximum binding sites for the addition of specific fatty acids for cell culture applications. Additional applications include protease-sensitive immunoassays such as RIA, EIA, fluorescent and chemiluminescent assays; as a protein standard, diluent; as a protein, conjugate or enzyme stabiliser; for hybridisation, nucleic acid-based assays; in fatty acid sensitive cell culture systems such as CHO, Vero and MDCK cells.
BSA is obtained by a proprietary heat-shock method, designed to prevent the excessive use of denaturing organic solvents. It undergoes a thermal inactivation during 3 hours at 65ºC in order to inactivate potential viruses. Lyophilised BSA has been 0.2µm filtered prior to being freeze-dried.
Bovine plasma used for the manufacture of this product is collected from French healthy cattle declared free from BSE disease in its clinical forms. It is sourced in EU-approved French slaughterhouses.
This product has met the following criteria: