Insect-XPRESS™ Medium is a protein-free formulation designed to support the growth of insect cell lines derived from Spodoptera frugiperda (Sd9 and Sf21). Cell densities in excess of 8.3 x 106 cells/ml can be achieved with suspension cultures of Sf9 cells using Insect-XPRESS™ Medium and an excess of oxygen. This formulation can also be used for stationary mono-layer cultures and shake-flask cultures. Insect-XPRESS™ Medium contains L-glutamine and supports superior production of recombinant proteins when using the Baculovirus Expression Vector System (BEVS). For cryopreservation of insect cells, Insect-XPRESS™ can be mixed 50:50 with Cryoprotective Medium.