Application

Ultrafiltration with 100,000 Dalton molecular weight cutoff Ultracel^® membrane. Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms. Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC. Concentration of dilute or pre-purified proteins from column eluents. Desalting, buffer exchange and protein dialysis. Size exclusion and protein fractionation.

Features and Benefits

Ultracel^® regenerated cellulose membrane with 100 kDa NMWCO delivers retentate recovery of greater than 90%. Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes). Heat-sealed membrane minimizes downstream extractables. 100% integrity tested for reliable performance. Convenient sample monitoring with translucent housing and volume gradations. Direct pipette sample access eliminates processing step to recover concentrate. High concentration factors of 80–100X.

General description

Amicon^® Ultra 4 mL centrifugal devices with Ultracel^® 100,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, IgG (156 kDa) at 1 mg/ml initial concentration is retained at >95% . The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon^® Ultra 15 mL filters deliver the highest recovery with short spin times.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany