Application

Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel^® membrane Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC Desalting, buffer exchange, and protein dialysis

Features and Benefits

Ultracel^® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90% Concentrates 500 µL down to 15 µL Concentration factor 25x to 30x High sample recovery (greater than 90% of dilute starting solution) Fast processing time: 10 to 30 minutes Reverse spin capability provides consistent recoveries

General description

Amicon^® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95% .The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.Amicon^® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon^® Ultra 0.5 mL filters deliver great performance and lower spin times.

Legal Information

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany

ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Color Code: Clear