Application

Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.Antifoam 204 has been used:in the expression and purification of PUL (PLAP,Ufd3p, andLub1p)domainin fed-batch fermentationto prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growthin the batch fermentation with mineral medium

Components

Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.

General description

Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

Other Notes

Contains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly.

Preparation Note

For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.