Application

Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

2x YT medium has been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

Components

16 g/L Tryptone10 g/L Yeast Extract5 g/L NaCl

Features and Benefits

2xYT powder provides: Standard formulation An inexpecnsive alternative to liquid

General description

2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Packaging

250 g in poly bottle

Reconstitution

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).