Application

Yeast Nitrogen Base With Amino Acids has been used:in the ZYP-31 media preparation for E.coliin synthetic dextrose (SD) medium to evaluate the influence of nitrogen sourceto culture Candida utilis for the production of ¹³C-labeled yeast extract as internal standardin the preparation of imaging mediumSuitable for use in classifying yeasts based on carbon requirements. Also suitable for general growth of yeasts with the addition of a carbon source (usually glucose).Yeast nitrogen base with amino acids is a satisfactory nitrogen source for general growth media. Contains all essential nutrients required for the growth of yeasts except carbohydrate.

Components

Component, amount (µg/L unless indicated)Nitrogen Sources:Ammonium sulfate, 5.0 g/LAmino Acids:L-histidine, 10 mg/LDL-methionine, 20 mg/LDL-tryptophan, 20 mg/LVitamins:Biotin, 2.0Calcium pantothenate, 400Folic acid, 2.0Inositol, 2.0 mg/LNicotinic acid, 400p-Aminobenzoic acid, 200Pyridoxine HCl, 400Riboflavin, 200Thiamine HCL, 400Trace Elements:Boric acidCopper sulfatePotassium iodideFerric chlorideMagnesium sulfateSodium molybdateZinc sulfateSalts:Potassium phosphate monobasic, 1.0 g/LMagnesium sulfate, 0.5 g/LSodium chloride, 0.1 g/LCalcium chloride, 0.1 g/L

General description

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.

Packaging

250 g in poly bottle

Reconstitution

Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.