Agarose, Type I-A, low EEO

Code: a0169-1kg D2-231

Analysis Note

The following is a list of properties associated with our agaroses:Sulfate content - used as an indicator of purity, since sulfate is the major...


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£1,620.00 1KG
£1,944.00 inc. VAT

Analysis Note

The following is a list of properties associated with our agaroses:Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.Gel strength - the force that must be applied to a gel to cause it to fracture.Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

Agarose has been used:as a component in the preparation of tumor tissue to embed the cell materialin the sample preparation for quantum dot analysisin the preparation of agarose-solidified medium to study the effect of medium solidification on the culture of isolated ovules

Biochem/physiol Actions

Agarose is used as bioink, because of its characteristic properties such as, biocompatibility, mechanical strength and gelling ability at low temperature.

General description

Agarose, a natural polymer is obtained from sea algae. This neutral linear polysaccharide is made up ofd-galactose and 3,6-anhydro-l-galactose. These are connected alternatively with the help of glycosidic bonds. Agarose has high significance in gel electrophoresis and gel chromatography.

Packaging

10, 25, 50, 100, 250, 500 g in poly bottle

1 kg in poly bottle

anion tracessulfate (SO42-): ≤0.20%
ash≤0.5%
EEO0.09-0.13
formpowder
gel strength≥1200 g/cm2 (1% gel)
impurities≤7% water
InChI keyMJQHZNBUODTQTK-WKGBVCLCSA-N
InChI1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
mp86.5-89.5 °C
Quality Level200
transition tempgel point 36 °C ±1.5 °C (1.5% gel)
turbidity≤4 NTU (1.5% gel)
typeType I-A
Code
Description
Unit Size
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Qty
A0169-100G
Unit:100G
List Price: £241.00
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A0169-10G
Unit:10G
List Price: £41.50
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A0169-250G
Unit:250G
List Price: £493.00
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A0169-25G
Unit:25G
List Price: £89.70
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A0169-500G
Unit:500G
List Price: £822.00
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A0169-50G
Unit:50G
List Price: £159.00
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Cas Number9012-36-6
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