Application

Casein Blocking Buffer 10x has been used in:western blotting enzyme linked immunosorbent assay (ELISA)immunohistochemistry

Features and Benefits

Better blocking performance than dried milk or BSA Easy-to-use concentrate Compatible with neutral and positively-charged membranes Compatible with chemiluminescent and colorimetric detection assays

General description

Sigma’;s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.