MyTaq™ HS DNA Polymerase is a high performance PCR product powered by antibody mediated hot-start, specifically designed for fast, highly-specific, hot-start PCR. The product also has the added convenience of room temperature reaction assembly, without non-specific amplification and primer-dimer formation. This new hot-start enzyme preparation from Meridian Bioscience is supplied with 5x MyTaq Reaction Buffer, a proprietary formulation containing dNTPs, MgCl2 and enhancers at optimal concentrations, removing the need for optimization and giving superior amplification.

Features

• New generation of antibody-based hot-start polymerase
• Highest specificity and superior performance
• Novel buffer system, including dNTPs and MgCl2
• Fast PCR reactions
• Applications: high-throughput PCR, assays with prolonged reaction setup on the bench or liquid handling, amplification of challenging targets susceptible to mispriming, amplification from challenging plant samples, colony PCR, multiplexing specific amplification of difficult templates (GC rich), genotyping, TA cloning, two-Step RT-PCR
• Components: MyTaq HS DNA Polymerase, 5x MyTaq Reaction Buffer
• Unit Definition: One unit is defined as the amount of enzyme that incorporates 10nmoles of dNTPs into acid insoluble form in 30 minutes at 72°C
• Concentration: 5u/µl
• Storage Conditions: MyTaq HS DNA Polymerase can be stored for up to 6 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.