The ECM 830 is a versatile electroporation system suitable for applications for gene, drug and protein delivery including: CRISPR, mammalian cells, in vivo, ex vivo, in ovo tissue, ex plant and nuclear transfer. The ECM 830 is a square wave pulse generator designed for in vitro and in vivo applications. The versatility of the ECM 830 applications for gene, drug and protein delivery include; mammalian cells, in vivo, ex vivo tissue, zebrafish tissue and embryos, nuclear transfer, embryo manipulation, plant protoplast and basic bacteria and yeast transformations. The ECM 830 can be used in combination with a wide array of BTX specialty electrodes and accessories to enhance your molecular and drug delivery experiments. This instrument is intended for research and pre-clinical use only and is not for use in diagnostic or clinical procedures.

High Throughput (HT) electroporation (45-0666 only): offers the advantage of multi-well electroporation technology for processing multiple samples in seconds. This permits a large number of samples to be quickly electroporated using high throughput plate handler technology. Using the HT Multi-Well plates instead of traditional cuvettes, the researcher can transition from a single cuvette to either a 96-well or 25-well electroporation plate by using a plate handler. This increases yields and the number of experiment runs in a single day. Experiments take seconds to run, allowing for quick and efficient optimization of the electrical and biological parameters. Once optimized, samples are rapidly processed increasing yields and saving valuable time and money. The key to the HT system is the combination of the ECM 830 generator with the plate handler and multi-well plates. The plate handler holds the plates and delivers the pulse(s) to the wells using parameters set in the ECM 830 generator.

Features:

• A wide range of voltages from 5 to 3000V
• Pulse durations from 10µsec to 999ms
• Pre-pulse resistance check, over-current pulse abort, and instrument arc protection safety features
• Easy to use touchscreen user interface
• Logs of every pulse delivered for QC and troubleshooting

In vivo applications:

• Mammalian cell transfection
• In vitro, in vivo, ex vivo and in ovo tissue transfection
• Nuclear transfer
• Ex plant tissue transfection
• Plant protoplast transfection
• siRNA libraries
• High throughput 25- and 96-well electroporation

Contents:

• 452052: ECM 830 generator, safety dome, 30 x cuvettes (10 x 1mm, 10 x 2mm, 10 x 4mm), and cuvette rack 660
• 450661: ECM 830 generator, 630B safety stand, 30 x cuvettes (10 x 1mm, 10 x 2mm, 10 x 4mm), and cuvette rack 660
• 450662: ECM 830 generator only
• 450666: ECM 830 generator, safety dome, 30 x cuvettes (10 x 1mm, 10 x 2mm, 10 x 4mm), cuvette rack 660, 2 x 96 well plates (4mm gap), plate seals, HT-200 plate handler, and plate adaptor
• 450669: ECM 830 generator, pulse switcher, power supply and USB connector cable, safety stand, 30 x cuvettes (10 x 1mm, 10 x 2mm, 10 x 4mm), and cuvette rack