Application

Analytes: ACTH, Corticosterone, Melatonin Recommended Sample Type: Rat serum, plasma, cell/tissue supernatants and lysates NOTE: For optimal assay performance of turbid serum and plasma samples an extraction protocol is provided. Recommended Sample Dilution: 25µL per well is used for the following: undiluted extracted serum or plasma; 1:4 non-extracted serum or plasma; cell/tissue culture samples may require dilution in an appropriate control medium. Assay Run Time: Overnight (16-18 hours) at 2-8°C Research Category: Endocrine Research Subcategory: Metabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Stress is defined as the disruption of homeostasis through physical and/or psychological stimuli orstressors. Physiologically the neuroendocrine system, which includes the hypothalamus, pituitary and adrenal glands, plays a critical role in response to stress. Activated by corticotropin-releasing hormone (CRH) and arginine-vasopressin (AV), the pituitary gland releases ACTH which results in the secretion of cortisol / corticosterone and other glucocorticoids from the adrenal glands. These corticoids involve the whole body in the stress response and are involved in the termination of the stress response by a negative feedback loop to the hypothalamus. Corticosterone and melatonin are important in the regulation of the sleep-wake cycle.MILLIPLEX^® Rat Stress Hormone Panel is a 3-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue/cell extracts or culture media samples: ACTH, corticosterone and/or melatonin. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Endocrine

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Please contact Technical Service for linearity of dilution.

Specificity

Cross ReactivtyThis assay display insignificant cross-reactivity within the panel