Application

Analytes: IgG1, IgG2, IgG3, IgG4, IgA, IgM Recommended Sample type: Serum, plasma, or tissue/cell lysate and culture supernatant samples Recommended Sample dilution: 1:16,000 diluted plasma or serum, or cell culture supernatant diluted to approximately 1µg/mL Ig per well Assay Run Time: One day Research Category: Inflammation & Immunology

Components

MILLIPLEX™ MAP Anti-Human κ and λ Light Chain, PEMILLIPLEX™ MAP Human Multi-Immunoglobulin StandardMILLIPLEX™ MAP Human Immunoglobulin Positive ControlMILLIPLEX™ MAP Assay BufferMILLIPLEX™ MAP Wash Buffer, 10X

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

General description

Produced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens, and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Five major isotypes have been identified in placental mammals: IgM, IgG, IgA, IgE and IgD (B-cell receptor) – all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions, and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes. In addition, isotyping applications include analyzing hybridomas during antibody generation and vaccine development. The MILLIPLEX^® Human Isotyping Bead Panel, measuring human IgG subclasses (1, 2, 3, 4), IgM and IgA is designed to enable you to measure both classes and subclasses accurately – all in one well. In addition, this panel allows you to choose only those immunoglobulins you need for your experiments. The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Immune Response

Legal Information

xMAP is a registered trademark of Luminex Corp

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany