Azocasein, protease substrate

Code: a2765-10g D2-231

Application

Azocasein has been used as a substrate for determination of protease activity.

Azocasein is an inflammatory agent that is used to induce amyloid A amylooido...


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Your Price
£180.00 10G
£216.00 inc. VAT

Application

Azocasein has been used as a substrate for determination of protease activity.

Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.

General description

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Linkage

View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer

Packaging

1, 5, 10, 25 g in glass bottle

ε (extinction coefficient)≥25 at 440 nm in 0.1 M NaOH at 1%
biological sourcebovine
formpowder
Quality Level200
solubilitywater: 5 mg/mL, clear, orange to very deep orange
storage temp.2-8°C
technique(s)ligand binding assay: suitable
Code
Description
Unit Size
List Price
Qty
A2765-1G
Unit:1G
List Price: £43.40
Source:List Price
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A2765-25G
Unit:25G
List Price: £449.00
Source:List Price
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A2765-5G
Unit:5G
List Price: £105.00
Source:List Price
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Cas Number102110-74-7
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