Application

•2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).†•ABTS is also used to measure glucose concentrations in different solutions including blood serum.•The food industry uses ABTS to measure the antioxidant capacities within different foods. •Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results •It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.

2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

General description

ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures.ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used:•For a reaction with laccase or bilirubin oxidase•For a reaction with peroxidase enzymes: (like HRP for example). ABTS in combination with hydrogen peroxide A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound

Reconstitution

Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 µl of fresh 30% hydrogen peroxide