Application

3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate, Supersensitive, for ELISA has been used as a substrate for peroxidase:to determine synthesized hyaluronan and HAS2 (hyaluronan-synthesizing enzyme) expression by the transfectantto measure the levels of α-hemolysin toxinin imaging bound IgGs

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 655 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm.

General description

This product contains 3, 3′,5 ,5′-Tetramethylbenzidine (TMB) in a mildly acidic buffer. The substrate is supplied as a one component ready to use solution. Rate kinetics are approx. 40% faster than traditional TMB formulations. Not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.