Application

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Biochem/physiol Actions

Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Other Notes

Chemoenzymatic synthesis of (-)-carbocyclic 7-deazaoxetanocin G

Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 µmol oleic acid per minute at pH 8.0 and 40 °C (triolein, Cat. No. 62314 as substrate)