Application

Carbonic Anhydrase has been used:as a component of nine-protein mixture for the comparative proteomic mass spectrometry analysis of bovine brain samplesas a molecular marker in gel filtration chromatographyas a standard protein to test the recovery of CMA/20 microdialysis probe

Biochem/physiol Actions

Carbonic anhydrase is essential for the reversible hydration of carbon dioxide. It is also crucial for the physiological processes including respiration, ion transport, acid–base balance, lipid and carbohydrate metabolic pathways. Carbonic anhydrase participates in electrolyte secretion and in the carbon dioxide fixation.

General description

Carbonic Anhydrase is a metalloenzymewith zinc. The active site of carbonic anhydrase contains three conserved histidine residues (His94, His96 and His119).

Unit Definition

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 µmole of leucine in 5 hours at pH 7.4 at 37 °C.