Application

Citrate Synthase from porcine heart has been used:to inject newt egg for determining its importance in egg activation at fertilizationin the preparation of reaction mix to determine pyruvate carboxylase enzyme activityto examine whether it can induce a [Ca²⁺]increase and egg activation in unfertilized eggs

Biochem/physiol Actions

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H₂O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 7.0.

Preparation Note

Dissolves in water to form a clear solution at 1 mg/mL concentration.

Unit Definition

One unit will form 1.0 µmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.