His Spintrap, Cytiva, 28-4013-53

Code: ge28-4013-53 D2-232

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Features and Benefits

• For simple, smal...


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Your Price
£393.00 PK50
£471.60 inc. VAT

Analysis Note

To view the Certificate of Analysis for this product, please visit www.gelifesciences.com.

Features and Benefits

• For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.• Ni Sepharose® High Performance in prepacked spin columns.• Yields up to 750 µg pure protein per column.• Allows direct purification of both clarified and unclarified cell lysates.

General description

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)* See the section for licensing information in our terms and conditions.Purification of unclarified samples on His SpinTrap columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.His SpinTrap columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap together with buffers prepared using His Buffer Kit.The columns are prepacked with Ni Sepharose® High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose® High Performance medium is negligible.

Legal Information

SpinTrap is a trademark of GE Healthcare

Sepharose is a registered trademark of GE Healthcare

Storage and Stability

Store at 4 to 30 °C (20% Ethanol)

Store at 4 to 30 °C (0.15% Kathon CG)

packagingpkg of 50 x 100 µL
avg. part. size34 µm
bed volume100 µL
shelf lifePlease be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
cleaning2 - 14 (Ni2+-stripped medium.)
working range3 - 12 (Ni2+-stripped medium.)
mfr. no.GE Healthcare, 28-4013-53
capacity≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
matrixhighly cross-linked 6% agarose
This product has met the following criteria: