Meridian Bioscience Ribonuclease Inhibitor (RiboSafe RNase Inhibitor) is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C by binding non-covalently in a 1:1 ratio (Fig. 1 & 2). RiboSafe shows no inhibition of polymerase or transcriptase activity (Fig. 4) and it is not effective against RNase H, T1, S1-nuclease or RNase from Aspergilus. With an association constant of 1014M, RiboSafe is useful in any applications where the presence of RNases is a potential problem. RiboSafe RNase Inhibitor is tested for activity, SDS-PAGE purity, and the absence of endonucleases, nickases and exonucleases. The enzyme is supplied at a concentration of 40u/µl.

Features

• Complete inhibition of RNase A, B and C
• Significantly increases RT-PCR sensitivity
• DNase/RNase and Nickase free
• No inhibition of polymerase/transcriptase activity
• Stable over a wide range of pH, temperatures and DTT concentrations

Applications

• RNA purification
• cDNA preparation by reverse transcription
• RNA sequencingin vitro RNA transcription
• in vitro protein synthesis

Storage Conditions RiboSafe RNase Inhibitor can be stored for 6 months at -20°C.