Application

Chirobiotic V HPLC column may be used for separating the enantiomers of citalopram and its two N-demethylated metabolites, and of the internal standard, alprenolol, in human plasma.

General description

Astec CHIROBIOTIC^® CSPs (chiral stationary phases) interact with ionisable, polar and neutral analytes through multiple molecular interactions. This property makes it widely versatile, thereby making it applicable in a variety of mobile phases. Another feature is the presence of ionic interactions, allowing them to be used reversed-phase and polar ionic modes for sensitive LC-MS operation. CHIROBIOTIC^® V is based on bonding Vancomycin which contains 18 chiral centers surrounding three cavities or pockets. These cavities are surrounded with five aromatic rings. Hydrogen acceptor and donor sites are present very near to the ring structures. The CHIROBIOTIC^® V is similar to glycoprotein phase′s selectivity wise, with its exception of exhibiting high sample capacity and being stable from 0-100% organic modifier.

Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC^® V in the polar ionic mode. The CHIROBIOTIC^® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC^® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity. Bonded phase: Vancomycin Operating pH range: 3.5 - 7.0 Particle diameter: 5, 10 or 16 µm Pore size: 100 Å (CHIROBIOTIC^® V) or 200 Å (CHIROBIOTIC^® V2)

Legal Information

CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Astec is a registered trademark of Sigma-Aldrich Co. LLC

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