SUPELCOSIL LC-Diol HPLC Column, 5 mum particle size, L x I.D. 25 cm x 4.0 mm

Code: 58201c40 D2-231

General description

LC-Diol columns can be used to separate proteins by gel filtration chromatography. When operated with an aqueous buffer, the diol phase can effectively sh...


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General description

LC-Diol columns can be used to separate proteins by gel filtration chromatography. When operated with an aqueous buffer, the diol phase can effectively shield the silica surface from interacting with proteins. A well-known use of diol columns, under normal phase conditions, is the separation of steroids and sterols.

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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agencysuitable for USP L20
application(s)food and beverages
extent of labeling3.5% carbon loading
featureendcapped: no
L × I.D.25 cm × 4 mm
manufacturer/tradenameSUPELCOSIL™
materialstainless steel column
matrix active groupdiol phase
matrixfully porous particle, silica gel, spherical particle platform
packaging1 ea of
parameter≤70 °C temp. range, 400 bar pressure (5801 psi)
particle size5 µm
pH range2-7.5
pore size120 Å pore size
product lineSUPELCOSIL™
Quality Level100
separation techniquenormal phase, hydrophilic interaction (HILIC), reversed phase
surface area170 m2/g
surface coveragesurface coverage 3.8 µmol/m2
technique(s)HPLC: suitable
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