BIOSHELL PROTEIN C18 3.4UM 2CM X 3.0MM

Code: 67471-u D2-231

Application

UHPLC Analysis of Proteins on BIOshell™ A160 Peptide C18 2.7 µm; BIOshell™ A400 Protein C18, 3.4 µm; and BIOshell...


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Application

UHPLC Analysis of Proteins on BIOshell™ A160 Peptide C18 2.7 µm; BIOshell™ A400 Protein C18, 3.4 µm; and BIOshell™ IgG 1000 Å C18, 2.7 µm

General description

BIOshell™ 400 Å Protein C18 columns contain 3.4 µm advanced Fused-Core® particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins. These columns offer maximum hydrophobicity for resolving tricky hydrophilic and PEGylated proteins. The 3.4 µm Fused-Core particles allow a shorter analysis time yet similar back pressure to fully porous 1.7 µm, 300 Å fully porous C18 particles. These columns can be operated long term at pressures up to 9000 psi (600 bar), temperatures up to 90 °C, and within the pH range of 1 to 9.

Legal Information

BIOshell is a trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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agencysuitable for USP L1
analyte chemical class(es)proteins
descriptionShell Thickness (0.2 µm), Solid Core (3.0 µm)
extent of labeling1% carbon loading
featureendcapped
L × I.D.2 cm × 3 mm
manufacturer/tradenameBIOshell™
materialstainless steel hardware (column)
matrix active groupC18 (octadecyl) phase
matrixsuperficially porous particle, spherical silica particle platform
operating pH1-8
packaging1 ea of
parameter90 °C max. temp., 600 bar max. pressure
particle size3.4 µm
pore size400 Å, 3.4 µm particle size
product lineBIOshell™
Quality Level100
separation techniquereversed phase
surface area~15 m2/g
technique(s)UHPLC-MS: suitable, HPLC: suitable, LC/MS: suitable, UHPLC: suitable
This product has met the following criteria: