Application

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

2′-Deoxycytidine 5′-triphosphate disodium salt has been used:in polymerase chain reaction mix for the amplification of Alternaria (AM) toxin DNAto spike oligonucleotide sample for capillary electrophoresis (CE) analysisas a component in adenosine 5′-monophosphate (AMP) luciferase assay

Biochem/physiol Actions

2′-Deoxycytidine 5′-triphosphate (dCTP) is used to synthesize DNA by DNA polymerases or reverse transcriptases. dCTP is an allosteric regulator of deoxycytidylate (dCMP) deaminase. It is used in studies of nucleotide pools, nucleotide selectivity and enzyme specificity studies, such as the preferred incorporation of dCTP by Rev1, a γ-family DNA polymerase.

General description

2′-Deoxycytidine 5′-triphosphate (dCTP) is synthesized either by de novo pathway or from the multiple phosphorylation steps of cytidine. It is deaminated to deoxyuridine monophosphate (dUMP) which is convertible to deoxythymidine triphosphate (dTTP).

Packaging

10 mg in glass bottle

1 g in poly bottle

25, 100 mg in poly bottle