Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Guanidine hydrochloride (GdnHCl) has been used: to perform absolute quantitation of amyloid-beta (Aß) isoforms in plasmain protein carbonylation assayfor the quantification of glycosaminoglycans (GAG)for denaturation of cow’;s milk allergensas a control in antiviral screening tests with coxsackievirus B3 (CVB3)in RNA isolation to dissociate nucleoproteins and inhibit RNase

Biochem/physiol Actions

Guanidine hydrochloride (GdnHCl) is hydroscopic and a small molecule. It is a potent denaturant of enzymes, which results in conformational changes in the enzyme, leading to complete loss of enzyme activity. GdnHCl is involved in blocking the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It is involved in denaturing alkaline phosphatase (ALPase) in Haliotis diversicolor. GdnHCl leads to the deactivation of enzymes like papain and aminoacylase. GdnHCl, at higher concentrations, is effective in virus inactivation, such as herpes simplex virus 1 (HSV-1).

Caution

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. For free-flowing material, please see our Redi-Dri^™ free-flowing product number RDD001.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Packaging

25, 100, 500 g in poly bottle

G4505-1KT:Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

2, 10, 25 kg in poly drum

1 kg in poly bottle

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes. The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.