Application

Aminoallyl-dUTP sodium salt has been used:to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesisfor the generation of chromatin immunoprecipitation (ChIP) probesin the reverse transcription of RNA for the synthesis of fluorescent labelled probes

Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.

Biochem/physiol Actions

Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.