Application

Guanidine thiocyanate has been used:as a denaturing agent to study its effects on surface activity of globular proteinfor the extraction of total RNA from primary hepatocytesas a component of the incubating buffer for extraction of DNA from human boneas a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNAas a component of lysis solution for extraction of RNA from rat pancreatic tissues

Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.

Packaging

25, 100, 250 g in poly bottle

500 g in glass bottle

1 kg in glass bottle