Application

CPRG has been used as a substrate for β-galactosidase assay.

Colorimetric determination of β-Gal activity in cell extracts and ELISA.

Biochem/physiol Actions

Chlorophenol red-β-D-galactopyranoside (CPRG) might possess oestrogenic activity.

General description

Chlorophenol red-β-D-galactopyranoside (CPRG) is a long-wavelength dye which is commonly used for colorimetric assays. The reaction product has a dark red color and is soluble in water. It can be evaluated photometrically at 574 or 578 nm.The chemical formula on the label represents the monosodium salt.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 5 mmol/lStorage conditions (working solution): Reconstituted solution is stable for one day at 2 to 8 °C

Quality

Contaminant: ﹤0.1% chlorophenol red

Sequence

CPRG is an approximately 1:1 mixture of the isomers.

Storage and Stability

Store powder dry at -15 to -25 °C; stock solutions at 2–8 °C protected from light.