Application

Monoclonal antibody for detection of both wild-type and mutant forms of GFP or GFP fusions using: ImmunoprecipitationWestern blotsImmunostaining

Features and Benefits

ContentsMixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200µg of total Anti-GFP IgG. Anti-GFP is a mixture of two clones (7.1 and 13.1).

General description

Green Fluorescent Protein (GFP) is a spontaneously fluorescent 27kDa protein originally isolated from the jellyfish Aequorea victoria. The molecular cloning of the GFP gene and its subsequent expression in heterologous systems has established GFP as a valuable reporter molecule for in vivo visualization of gene expression events in a wide variety of cell types and organisms. Since, GFP requires no additional substrates or cofactors, GFP’;s green fluorescence can be easily detected using blue or UV light after expression in either prokaryotic or eukaryotic cells. In addition, several mutant forms of GFP with unique spectral properties (e.g., enhanced fluorescence signal and shifts in excitation and emission spectra) have been reported.

Mixture of two high-affinity monoclonal antibodies selected for their performance in detection of GFP and GFP fusion proteins.

Legal Information

This product is sold under license from Columbia University. Rights to use this product are limited to research use only. No other rights are conveyed. Inquiry into the availability of a license to broader rights or the use of this product for commercial purposes should be directed to Columbia Innovation Enterprise, Columbia University, Engineering Terrace - Suite 363, new York, New York 10027.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Working concentration of antibody depends on application and substrate.The following concentrations should be taken as a guideline: Western blot: 1:1000 dilution Immunoprecipitation: 2 to 10 µgStorage conditions (working solution): -15 to -25 °C

Quality

Anti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation.Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses.

Reconstitution

Add 500 µl double distilled water to a final concentration of 0.4 mg/ml.Rehydrate on ice for 30 minutes.

Specificity

Subtype: Both clones are Mouse IgG₁κ