Application

cOmplete^™ Protease Inhibitor Cocktail has been used as an component in extraction buffer for protein extraction from Arabidopsis seedling in radioimmunoprecipitation assay (RIPA) buffer for the isolation of protein from lymphocytes the extraction buffer for the lysis of protoplast.

cOmplete^™ is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.cOmplete contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete to all stock buffers and solutions normally protected with protease inhibitors, and not just during the initial purification steps.cOmplete contains EDTA (1 tablet yields a 1 mM EDTA solution in 50 ml). Therefore, the extraction buffer should not contain divalent cations like Ca²⁺, Mg²⁺, or Mn²⁺, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography. Alternatively, the product cOmplete, EDTA-free, can be used. These tablets are identical to cOmplete, with the exception that no EDTA or other chelating agent is present.cOmplete Tablets inhibit proteolytic activity in large extraction volumes (up to 50 ml). If you are working with smaller volumes (up to 10 ml), we recommend to use cOmplete, Mini. The composition of the cOmplete, Mini Tablet, is identical to the normal cOmplete Tablet.

Components

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Features and Benefits

Use cOmplete Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results. Convenience: Simply push the quick-dissolving tablet through the foil packaging of the EASYpack into your buffer. Complete protection: Instantly protect your proteins against a broad range of proteases. Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi. Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.

General description

cOmplete Protease Inhibitor Tablets inhibit a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains. Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.cOmplete tablets contain EDTA and both irreversible and reversible protease inhibitors. Protease inhibitors present in cOmplete Tablets do not form irreversible complexes with the SH groups of proteins.

Legal Information

cOmplete is a trademark of Roche

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

20 individually packed cOmplete Protease Inhibitor Cocktail Tablets in foil blisters.

Physical form

Tablets in foil blister packs

Preparation Note

Working concentration: 1 tablet per 50ml extraction solutionWorking solution: HandlingCarefully push the tablet through the foil packaging using the base of your thumb (not finger nail) to prevent the breakage of tablets.Preparation of Working SolutionOne cOmplete tablet is sufficient for the inhibition of the proteolytic activity in 50ml extraction solution. When a very high proteolytic activity is present, one tablet should be used for 25ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (25 X conc.) can be prepared.Preparation of Stock solution (25 X conc.)Dissolve one cOmplete tablet in 2ml dist. water or in 2ml 100 mM phosphate buffer, pH 7.0.Storage conditions (working solution): The stock solution can be stored at 2 to 8°C for 1 to 2 weeks, or at least 12 weeks at -15 to -25°C.

Reconstitution

Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

Specificity

Inhibits a broad spectrum of serine proteases, cysteine proteases, and metalloproteases, as well as calpains.

Storage and Stability

Store at 2 to 8 °C. (storage at -15 to -25 °C is also possible)