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MyTaq™ HS Red Mix is a ready-to-use 2x mix for fast, highly-specific hot-start PCR. MyTaq HS Red Mix is powered by antibody mediated hot-start and does not possess polymerase activity during the reaction set-up, thus reducing non-specific amplification. The advanced formulation of MyTaq HS Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity and yield , making it ideal for high-throughput. MyTaq HS Red Mix contains all the reagents including MyTaq buffer, dNTPs, MgCl2, enhancers and stabilizers necessary for trouble-free PCR reaction set up. The product is supplied conveniently all-in-one tube to reduce the number of pipetting steps and to facilitate increased efficiency, throughput and reproducibility. This makes MyTaq HS Red Mix perfect for techniques such as for multiplex PCR that require the ability to define a set of common reaction parameters to all of the primer sets in the reaction, with comparable extension efficiencies, even though the amplicons are different sizes . MyTaq HS Red Mix enables success in multiplex PCR at the first attempt and can even be used for fast multiplex PCR, without special kits, buffers or optimization. The specially designed MyTaq Red formulation does not interfere with the PCR reaction and allows users to load samples directly onto a gel after the PCR without the need to add loading buffer, making MyTaq HS Red Mix ideal for automation. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose). MyTaq HS Red Mix only requires the addition of template, primers and water, reducing the risk of pipetting errors and contamination as well as reducing the set-up time.

Features

• Convenient all-in-one mastermix
• New generation of antibody-based hot-start polymerase
• Highest specificity and superior performance
• Fast PCR reactions
• Red dye for direct gel loading

Applications

• High-throughput PCR
• Assays with prolonged reaction setup on the bench or liquid handling
• Amplification of challenging targets susceptible to mispriming
• Multiplexing
• Colony PCR
• Specific amplification of difficult templates (GC rich)
• Genotyping
• TA cloning
• Two-Step RT-PCR

Components: MyTaq HS Mix

Concentration: 2x

Storage Conditions: MyTaq HS Red Mix can be stored for up to 12 months at -20°C, or up to 2 weeks at +4°C. Repeated freeze/thaw cycles should be avoided.