The SensiFAST SYBR Hi-ROX One-Step Kit has been developed for fast RT-qPCR and has been validated on several commonly used real-time instruments. The SensiFAST SYBR Hi-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. The SensiFAST SYBR Hi-ROX One-Step Kit uses a combination of the latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, to ensure fast, highly-specific and ultra-sensitive one-step RT-qPCR . The SensiFAST SYBR Hi-ROX One-Step Kit consists of a 2x SensiFAST SYBR One-Step mix, which contains ROX for optional use, plus separate reverse transcriptase and RiboSafe RNase Inhibitor.

Volume BIO-73001: 100 x 20µl Reactions: 1 x 1ml BIO-73005: 500 x 20µl Reactions: 5 x 1ml

Features and Benefits

• Rapid - hot start capability saves time and reduces primer-dimer formation
• Unique buffer chemistry - for highest specificity and sensitivity
• Accurate quantification - from low copy targets
• Sensitive – unique buffer chemistry for highest specificity

Instrument Compatibility: ABI 7000, 7300, 7700, 7900, 7900HT, StepOne™, StepOne™ Plus, (See product selection table), each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

Components: 2x Reaction mix consisting of: Heat-activated DNA polymerase, Ultra-pure dNTPs, MgCl2, ROXSYBR® Green I, Plus separate vials of: Reverse transcriptase, RNase inhibitor, DEPC-treated water

Kit Size: The kit size is based on a 20µl final reaction volume.

Storage Conditions: 12 months at -20°C. Avoid exposure of the SYBR® Green I to light.