The SensiFAST™ Probe Hi-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan® , Scorpions®and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers fast, highly-specific and ultra-sensitive real-time PCR . SensiFAST Probe is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs, stabilizers and enhancers.

Features and Benefits

• Rapid - hot-start capability saves time and reduces primer-dimer formation
• Sensitive – perfect for hard to obtain and low copy number samples
• High performance - ideal for multiplexing and gene expression analysis
• Fast protocols - use with the new generation of fast cyclers

Instrument Compatibility: ABI 7000, 7300, 7700, 7900, 7900HT, 7900HT FAST, StepOne™, StepOne™ Plus (see product selection table) each of these instruments having the capacity to analyze the real-time PCR data with the passive reference signal either on or off, as well as several instruments that do not require the use of ROX.

Volume BIO-82002: 250 x 20µl Reactions: 2 x 1ml BIO-82005: 500 x 20µl Reactions: 5 x 1ml BIO-82020: 2000 x 20µl Reactions: 20 x 1ml

Components2x Reaction mix consisting of: Heat-activated DNA polymerase, Ultra-pure dNTPs, MgCl2, ROX Internal reference

Kit Size: The kit size is based on a 20µl final reaction volume.

Storage Conditions: 12 months at -20°C. Avoid exposure of the ROX™ to light.