The SensiFAST™ Probe No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly used real-time instruments. The kit has been formulated for use with probe-detection technology, including TaqMan®, Scorpions®and molecular beacon probes . A combination of the latest advances in buffer chemistry and PCR enhancers, together with a hot-start DNA polymerase, ensures that the SensiFAST Probe Kit delivers fast, highly-specific and ultra-sensitive real-time PCR. SensiFAST Probe is provided as a 2x mastermix containing all the components necessary for real-time PCR, including dNTPs, stabilizers and enhancers.

Features and Benefits

• Rapid - hot-start capability saves time and reduces primer-dimer formation
• Sensitive – perfect for hard to obtain and low copy number samplesHigh performance - ideal for multiplexing and gene expression analysis
• Fast protocols - use with the new generation of fast cyclers

Instrument Compatibility: Roche LightCycler® 480 platform, Bio-Rad iCycler®, iQ®5, MyiQ™, Opticon™, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler®ep realplex, Illumina® Eco™, Takara Thermal Cycler Dice® (TP800) and Techne Quantica®.

Volume BIO-86002: 250 x 20µl Reactions: 2 x 1ml BIO-86005: 500 x 20µl Reactions: 5 x 1ml BIO-86020: 2000 x 20µl Reactions: 20 x 1ml

Components: 2x Reaction mix consisting of: Heat-activated DNA polymerase, Ultra-pure dNTPs, MgCl2

Kit Size: The kit size is based on a 20µl final reaction volume.

Storage Conditions: 12 months at -20°C. Avoid exposure of the ROX™ to light.