Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Features and Benefits

For rapid purification of labeled DNA (>20 bases) from unincorporated labeled nucleotides in a volume of 25 to 50 µ using spin-column chromatography. Columns are prepacked with Sephadex^® G-50 Grade pre-equilibrated in TE buffer with 0.05% Kathon CG/ICP Biocide. Product recovery will be at least 10% lower than that from ProbeQuant^™ G-50 Micro Columns. Can also be used for buffer exchange/desalting of enzymatic reactions (10 to 100 µ) following heat denaturation and phenol extraction.

General description

MicroSpin^™ G-50 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions. Good product yield and purity is obtained with sample volumes from 12-50 ul. They are suitable for any DNA greater than 20 bases in length. They will not remove or denature enzyme.

Legal Information

MicroSpin is a trademark of Cytiva

ProbeQuant is a trademark of Cytiva

Sephadex is a registered trademark of Cytiva

Storage and Stability

Store at 20 to 25 °C. (TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.)