Application

SeqPlex^™ DNA Amplification Kit has been used for whole genome amplification.

Features and Benefits

Random priming technology amplifies fragmented DNA such as ChIP or FFPE Facilitates sequencing from as little as 100 pg of ChIP DNA Enhanced primers for complete genome coverage, minimal sequence bias, primer removal, and amplicon size ideal for next gen sequencing Compatible with Illumina^®, SOLiD^™, or 454 library prep for next generation sequencing

General description

SeqPlex^™ Enhanced DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA. The yields from chromatin immunoprecipitation (ChIP) or formalin-fixed paraffin-embedded tissue samples (FFPE) are often less than required for successful next generation sequencing library preparation. The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented DNA samples for input into a NGS workflow. This kit is an extension of the WGA product line and has been developed to integrate into the Illumina^®, SOLiD^™, or 454 sequencing workflows.

Legal Information

iCAT is a registered trademark of University of Washington

Illumina is a registered trademark of Illumina, Inc.

SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

Other Notes

SEQXE-500RXN is manufactured on-demand. Contact technical services at techserv@sial.com for more information.