Uracil DNA Glycosylase (UDG) catalyzes the release of uracil from uracil-containing single– or double-stranded DNA, but not from RNA or oligonucleotides (6 or fewer bases). UDG is active over a broad pH range with an optimum at pH 8.0, does not require a divalent cation, and is inhibited by high ionic strength (>200mM). Meridian Bioscience UDG is purified to SDS-PAGE purity and is free of endonucleases, exonucleases, nickases and RNases.

Applications

• UDG treatment of uracil-containing DNA to prevent amplification by DNA polymerases
• Used to investigate features of protein – DNA interactions
• Source: Recombinant E. coli strain carrying the over expressed gene of Uracil DNA Glycosylase from E. coli
• Components: Uracil DNA Glycosylase, 10x Reaction Buffer
• Unit Definition: One unit is the amount of enzyme that catalyzes the release of 60µmol of uracil per minute from uracil-containing double-stranded DNA
• Concentration: 1u/µl
• Storage Conditions Uracil DNA Glycosylase can be stored for 6 months at –20°C