The SensiFAST™ SYBR No-ROX One-Step Kit has been formulated for highly reproducible first-strand cDNA synthesis and subsequent real-time PCR in a single tube. A combination of the latest advances in buffer chemistry together with a reverse transcriptase and hot-start DNA polymerase system, ensures that SensiFAST SYBR No-ROX One-Step Kit produces fast, highly-specific and ultra-sensitive one-step RT-qPCR. The SensiFAST SYBR No-ROX One-Step Kit consists of a 2x SensiFAST SYBR One-Step mix, as well as separate reverse transcriptase and RiboSafe RNase Inhibitor.

Features and Benefits

• Rapid - hot start capability saves time and reduces primer-dimer formation
• Unique buffer chemistry - for highest specificity and sensitivity
• Accurate quantification - from low copy targets
• Sensitive – unique buffer chemistry for highest specificity

Instrument Compatibility: Roche LightCycler® 480 platform, Bio-Rad Opticon®, Opticon™2, Chromo4™, MiniOpticon™, CFX96™, CFX384™, Cepheid SmartCycler®, Qiagen Rotor-Gene™ 3000, 6000, Eppendorf Mastercycler® ep realplex and Techne Quantica®. (See product selection table).

Components: 2x Reaction mix consisting of: Heat-activated DNA polymerase Ultra-pure dNTPs, MgCl2, SYBR®Green I; Plus separate vials of: Reverse transcriptase, RNase inhibitor, DEPC-treated water

Kit Size: The kit size is based on a 20µl final reaction volume.

Storage Conditions: 12 months at -20°C. Avoid exposure of the SYBR® Green I to light.

Volume BIO-72001: 100 x 20µl Reactions: 1 x 1ml BIO-72005: 500 x 20µl Reactions: 5 x 1ml