Application

After 48 hours in medium, add fresh inhibitors in fresh medium.

Caution

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Components

AprotininBestatinE-64LeupeptinPepstatin A

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibtion of serine, cysteine, aspartic and aminopeptidases. Contains aprotinin, bestatin, leupeptin, E-64, and pepstatin A. Contains no metal chelators

Packaging

1 mL in glass bottle

Physical form

Solution in DMSO (D 2650, Hybri-Max).

Quantity

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Specificity

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases