Application

Catch and Release™ v2.0 should not be used with proteins containing a 6-His tag.

Components

Catch and Release™ Wash Buffer, 10X (Cat.# 20-210)Catch and Release™ Non-denaturing Elution Buffer, 4X (Cat.# 20-209)Catch and Release™ Denaturing Elution Buffer, 1X (Cat.# 20-284)20-28520-216Catch and Release™ Capture Tubes

General description

Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form



Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release™ is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

CATCH AND RELEASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

50 immunoprecipitations

1 kit in 50 immunoprecipitations

Quality

routinely evaluated by immunoprecipitation.

Storage and Stability

All components to be stored at 4°C except the Catch and Release™ Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.