Application

Prediluted for use in Immunohistochemical staining procedures. For example:

Blocking Reagent

Primary Antibody or Negative Control Reagent

Biotinylated Secondary Antibody

Streptavidin-Enzyme Conjugate

Chromogen

Counterstain

For best results use this reagent in conjunction with other IHC Select™ detection reagents. A complete list of reagents is available at www.chemicon.com.

Research CategoryAll

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

For use as a Pretreatment Reagent in Immunohistochemical staining procedures after dilution. Use prepared 1X Citrate Buffer to retrieve Epitopes in formalin-fixed, paraffin-embedded tissue sections mounted on glass microscope slides. This procedure is performed after deparaffinization and prior to IHC1,2.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid Citrate Buffer containing 0.1% ProClin 300 as a preservative.

Storage and Stability

When stored at 2-8°C, this reagent is stable up to the expiration date printed on the label. Do not freeze or expose to elevated temperatures.